Pharmacodynamics

The task of pharmacodynamics is to determine the drug mechanism of action at the molecular level and to analyze its physiological and biochemical effects on the body. Pharmacodynamic studies describes therefore the pharmacological effect of analyzed drug. Pharmacodynamics examines the processes such as binding of compounds by membrane receptors, regulation of enzyme activity in metabolic and regulatory pathways, signal transduction analysis by measurement of production of secondary messengers or protein phosphorylation, interaction of NCEs (New Chemical Entities) with cell membrane transporters, structural proteins as well as genetic material. Thus, pharmacodynamic studies enable determination of chemical structure of the drug with optimal Target Molecular Profile (TMP) and activity.


Selvita offers broad spectrum of pharmacodynamic studies used to analyze product?s molecular activity. Scope of analysis depends on the type of the product and is designed based on clear understanding of its characteristics and activity. Selvita offers assays that are able to detect even minor changes in biological response to pharmacologically active substances:

  • Analysis of key protein phosphorylation during the signal transduction
  • Analysis of receptor autophosphorylation (activation) upon biosimilar stimulation
  • Cell based functional assays (production of cAMP, an omnipresent cellular second messenger, which plays a key role in signal transduction after ligand binding to the GPCRs)
  • Protein biomarkers expression analysis (with the use of Western Blot or ELISA assays)
  • Characterization of protein-protein and drug-protein interactions (ELISA, fluorescence anisotropy)
  • Radiometric Assay Services